Department of Experimental Immunotherapy
The Department of Experimental Immunotherapy conducts advanced research and development activities focused on innovative therapeutic approaches. Our key areas of interest include:
- Development of selective biological agents of parasitic origin for the treatment of allergic, autoimmune, neurodegenerative, and oncological diseases. Our “learning from parasites” strategy utilizes in vitro, ex vivo systems, and animal models.
- Identification of infectious agents and immune mechanisms involved in the pathogenesis of neurodegenerative disorders.
- Discovery and validation of clinically relevant biomarkers for neurodegenerative diseases, aimed at improving patient qualification and predicting responses to therapy.
- Evaluation of the antiparasitic potential of naturally derived compounds against different developmental stages of intestinal nematodes.
Head of the Department
About the Laboratory
The Department has specialised laboratories with GMM Class II Genetic Engineering Department status and a Flow Cytometry Reference Laboratory.
Analysis of cell phenotype and activity, cytotoxicity:
- Flow cytometry (CytoFlex S cytometer, measurement of 13 fluorescence)
- Flow cytometry sorting (CytoFlex SRT sorter)
- Positive/negative separation method on magnetic beads
- ELISA, ELISPOT, immunoenzymatic assays (fluorescence, luminescence, colorimetry), MTT, MTS etc.
- Fluorescence microscopy
Imaging and microscopic characterisation of cells and sections:
- Light and fluorescence microscopy
- Immunohistochemistry of cells and tissues
Genomic and proteomic analysis:
- Single cell RNA sequencing (scRNA-seq)
- RT-PCR, dd-PCR
- 1D and 2D electrophoresis
- Western-blot
- Protein Array
- Reporter systems
In vivo models:
- Mouse model of multiple sclerosis (EAE)
- Mouse model of Crohn’s disease (TNBS)
- Mouse model of colitis ulcerosa (DSS)
- Mouse model of hookworm infection (Heligmosomoides polygyrus)
In vitro cultures of primary cells and cell lines (human, mouse, rat):
- Macrophages, T cells, dendritic cells
- Mast cell lines HMC 1.1./ HMC 1. 2/ RBL
- Dendritic cell line JAWSII
- T cells of lineage EL-4
- epithelial adenocarcinoma HeLa cell line
- epithelial adenocarcinoma Caco-2 cell line
- Haematological tumour cell lines: DLBCL (TMD8, U2932, DHL4, Ly1, Ramos, Raji), cHL (L428, SUP HD1, HDLM2), AML (KG1, MOLM14, MOLM16, Kasumi, HEL), T-ALL (Jurkat)
- Hepatocellular carcinoma HepG2 cell line
- epithelial Thle-2 cell line
